Production of Potent Fully Human Polyclonal Antibodies against Ebola Zaire Virus in Transchromosomal Cattle

Production of Potent Fully Human Polyclonal Antibodies against Ebola Zaire Virus in Transchromosomal Cattle

Polyclonal antibodies, derived from humans or hyperimmunized animals, have been used prophylactically or therapeutically as countermeasures for a variety of infectious diseases. SAB Biotherapeutics has successfully developed a transchromosomic (Tc) bovine platform technology that can produce fully human immunoglobulins rapidly, and in substantial quantities, against a variety of disease targets...

Production of potent fully human polyclonal antibodies against Zaire Ebola virus in transchromosomal cattle

Human Polyclonal Antibodies Produced through DNA Vaccination of Transchromosomal Cattle Provide Mice with Post-Exposure Protection against Lethal Zaire and Sudan Ebolaviruses

DNA vaccination of transchromosomal bovines (TcBs) with DNA vaccines expressing the codon-optimized (co) glycoprotein (GP) genes of Ebola virus (EBOV) and Sudan virus (SUDV) produce fully human polyclonal antibodies (pAbs) that recognize both viruses and demonstrate robust neutralizing activity. Each TcB was vaccinated by intramuscular electroporation (IM-EP) a total of four times and at each a...

Potent neutralizing monoclonal antibodies against Ebola virus infection.

Ebola virus infections cause a deadly hemorrhagic disease for which no vaccines or therapeutics has received regulatory approval. Here we show isolation of three (Q206, Q314 and Q411) neutralizing monoclonal antibodies (mAbs) against the surface glycoprotein (GP) of Ebola virus identified in West Africa in 2014 through sequential immunization of Chinese rhesus macaques and antigen-specific sing...

Emergence of Divergent Zaire Ebola Virus Strains

Gilda Grard,1 Roman Biek,2 Jean-Jacques Muyembe Tamfum,3,4 Joseph Fair,4 Nathan Wolfe,4 Pierre Formenty,5 Janusz Paweska,6 and Eric Leroy1,7 1Centre International de Recherches Médicales de Franceville, Gabon; 2College of Medical, Veterinary and Life Sciences and Medical Research Council– University of Glasgow Centre for Virus Research, University of Glasgow, United Kingdom; 3Institut National ...